The ChemiDoc MP system remains in focus at any zoom level. The ChemiDoc MP system makes it easy for researchers to acquire high-quality images in seconds, whether for chemiluminescence and fluorescence detection or for colorimetric gel and blot documentation. Using the ChemiDoc MP system, researchers can visualize protein separation on Criterion™ TGX Stain-Free™ Precast gels, verify successful transfer with the Trans-Blot ® Turbo™ transfer system by imaging the membrane using stain-free detection, and validate the accuracy of western blot results at every step. In addition, the V3 Western Workflow offers researchers confidence in their western blot results, providing them a way to visualize their proteins at every stage of the protocol. The ChemiDoc MP system also integrates with Bio-Rad's V3 Western Workflow™, a portfolio of Bio-Rad products that shorten the protein separation and blot transfer steps from several hours to 30 minutes. Furthermore, a published report indicates that normalizing against housekeeping proteins might produce inaccurate results, depending on experimental conditions and sample types.Īssurance at Every Stage of the Western Workflow Alternatively, probing with multiple antibodies simultaneously requires reagent optimization over several experiments. Stripping and reprobing adds extra steps to the protocol, consuming reagents, time, and labor. Researchers quantifying western blot experiments traditionally normalize data with housekeeping proteins, which often requires stripping and probing the membrane a second time, or probing with multiple antibodies simultaneously. "This also saves time and hassle since I don't need to reprobe the blot for housekeeping proteins." "I've found that using stain-free imaging with the ChemiDoc MP is as good as or better than using housekeeping proteins for normalization," said Aldrin Gomes, assistant professor at the University of California, Davis. This stain-free total protein normalization method produces faster results and overcomes the potential errors associated with normalization using housekeeping proteins. Using the stain-free detection option of the ChemiDoc MP system, researchers can quantify total protein for normalization purposes after transfer to the membrane. announces the launch of its ChemiDoc™ MP Imaging System, the industry's only stain-free enabled instrument capable of chemiluminescent, multiplex fluorescent, and routine gel imaging. Hercules, CA - SeptemBio-Rad Laboratories, Inc. ChemiDoc MP imaging system's stain-free technology helps speed the western blotting workflow.